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Plasmid pMLS-SV40-EGFP from Dr. Jonathan Weissman's lab contains the insert GFP and is published in Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044.
SV40 late control region DNA fragments, containing specific deletions, were (1 days ago) An SP6 promoter is present in the 5' untranslated region of the mRNA from the sCMV promoter, allowing in vitro RNA synthesis of sequences cloned into the polylinker. A T7 promoter in reverse orientation between the polylinker and the SV40 polyA site for probe synthesis, as well as a second polylinker after the SV40 polyA site to provide several 1988-05-20 · The SV40 promoter is expressed well in the fission yeast S. pombe, and it initiates transcription at the same site as in mammalian cells. The majority of the enhancer sequences, however, do not contribute to this activity. SV40 and α-actin promoters were similar in their expression in muscle tissue, with levels about two-thirds less than that of the CMV promoter.
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like CMV, EF1A, and SV40 promoters, are always active and thus referred to as constitutive promoters. Others are only active under specific circumstances. In this post, we’ll discuss inducible promoters , which can be switched from an OFF to an ON state, and how you might use these in your research. Plasmid pMLS-SV40-EGFP from Dr. Jonathan Weissman's lab contains the insert GFP and is published in Cell.
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• SV40 promoter for high-level constitutive expression of your gene of interest • CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Zeocin™ fusion • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the SV40 large T antigen SV40 / hAlb promoter (Liver) in pDRIVE expression plasmid.
They will not replicate episomally in the absence of the SV40 large T antigen. The SV40 promoter is expressed well in the fission yeast S. pombe, and it initiates transcription at the same site as in mammalian cells. The majority of the enhancer sequences, however, do not contribute to this activity. SV40 promoter is also fairly strong, though generally somewhat weaker than EF1A and CAGG. While there is cell type to cell type variability for all the promoters, CMV promoter is the most variable, being very strong in some cell types ( e.g. , 293T and CMMT) and rather weak in others ( e.g.
We report that the early transcriptional activity downstream of the SV40 promoter remains unaffected by CGGBP1 depletion.
The SV40 promoter is fairly weak in 293T cells compared to the CMV promoter. So for the highest expression, For the highest protein "overexpression",
SV40 is an abbreviation for simian vacuolating virus 40 or simian virus 40, a polyomavirus that is found in both monkeys and humans.Like other polyomaviruses, SV40 is a DNA virus that has the potential to cause tumors in animals, but most often persists as a latent infection.
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SV40 promoter. The SV40 (Simian Virus 40) promoter contains the SV40 enhancer promoter region and origin of replication (part no.
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Type 1 Interferons Promote a Diabetogenic Microenvironment
The underlined regions at the 5´ end of the fragment containing the SV40 promoter are the two 72 bp enhancer repeats. Promoters control the binding of RNA polymerase and transcription factors. Since the promoter region drives transcription of a target gene, it therefore determines the timing of gene expression and largely defines the amount of recombinant protein that will be produced.